Protein_Domain

Part:BBa_K4724004

Designed by: Qingqing Zheng   Group: iGEM23_NJTech-China-A   (2023-10-06)


CBM4-6xHis Tag

The hydrophobic structural domain CBM4 is linked to the protein, which can improve the hydrophobicity of the protein. Under the force of water molecule movement, PETase connected to hydrophobic domains will more easily bind to PET surfaces.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 295
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 216
    Illegal AgeI site found at 169
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None